The Discovery of DNA - 3 - The Hershey and Chase Experiment

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vittorio s

Published on Nov 28, 2024
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TEST YOURSELF with the Verification Test (does not require personal data or registration) https://forms.gle/TNK44bUzAGcSZRD26 At the end of the test you can view the answers you gave and see your score, correct and incorrect answers. Third of four videos on the discovery of DNA (third year high school program) Griffith's experiment and the subsequent one by Avery and collaborators had demonstrated with good certainty that it was DNA and not proteins that was the molecule responsible for genetic information. The resistance to this demonstration was difficult to dismantle, until the experiment by Hershey and Chase described in this video. The main objective of Hershey and Chase was therefore to determine which component of viruses, proteins or deoxyribonucleic acid (DNA), was responsible for transmitting genetic information to host cells. The two scientists managed to resolve the dilemma of whether the genetic material was made up of proteins or DNA. They used a very refined approach, using T2 Bacteriophages: viruses, which infect Escherichia coli bacteria and are made up of a protein capsid that wraps the genetic material (DNA). So they are only made up of DNA and proteins. To trace the DNA they used radioactive Phosphorus (³²P) and to trace the proteins they used radioactive Sulphur (³⁵S). Watch the video to find out how they did it. In the next video the last two series of data that led Watson and Crick to the discovery of the structure of DNA: Chargaff's rules and Rosalind Franklin's X-ray photography of DNA crystals. 00:20 presentation 01:40 T2 series phages 03:50 the mechanism of phage infection 04:45 the structure of T2 phages 06:00 the experimental procedure Previous videos Miesher and nuclein, Levene and nucleotides • The discovery of DNA - 1 - Miescher, the... Griffith and Avery's experiment • The discovery of DNA - 2 - the experimen... Next video: Chargaff's rules and Rosalind Franklin's photo • The discovery of DNA - 4 - The rules of... The chromosomal theory of inheritance and Morgan's experiments podcast: • Morgan and the chromosomal theory of inheritance and Morgan's experimen... Mendelian genetics: • Mendelian genetics DNA and gene expression: • DNA and gene expression https://scienzita.blogspot.com/

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