Quantitative PCR (qPCR, real-time PCR)
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Hello everyone, welcome to the Easy Biochemistry channel. In this video, we discuss the principle and applications of quantitative PCR (qPCR), also called real-time PCR. We discuss the differences between classic PCR and qPCR, including the increased accuracy of the latter thanks to real-time detection of amplification products. The video also covers the two main fluorescent markers used in qPCR: SYBR Green and the Taqman probe, as well as their operation and specificities. Some diagrams in this video were made using biorender.com (created with BioRender.com). If you liked the video, like, share and comment so that the video can help more people ❤ don't forget to subscribe :) Thank you! Other videos that might interest you: WHAT IS PCR? PRINCIPLE AND APPLICATIONS • What is PCR? Principle and Appl... MOLECULAR HYBRIDIZATION AND NUCLEIC PROBES • MOLECULAR HYBRIDIZATION AND NUCLEIC PROBES... PCR PRIMER DESIGN • PCR PRIMER DESIGN Tutorial ... Join me on Instagram: https://www.instagram.com/biochimie_f... See you soon!
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