Everything you need to know and understand about the ELISA method! (long version) - Bioteach - Watch - Learn and discover! - World Latest Movie, Series, Videos and Trailers | fetery.com

Everything you need to know and understand about the ELISA method! (long version)

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Bioteach - Watch - Learn and discover!

Published on May 12, 2023

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Subscribe to the channel now!!! / / @laurentmartorell • Short version available here: • Everything you need to know and understand about the meth... The ELISA (enzyme-linked immunosorbent assay) method is a technique commonly used in immunology and biochemistry to detect the presence of antibodies or antigens in a biological sample. It is based on the ability of antibodies to bind specifically to their corresponding antigen. The ELISA method uses microtiter plates that are coated with a specific antigen or an antibody that binds to the target antigen. When a sample containing the antigen is added to the plate, it binds to the antibodies immobilized on the surface of the plate. Unbound antibodies are then washed away. Then, enzyme-labeled antibodies are added, which bind specifically to the antibodies or antigens immobilized on the plate. After another wash to remove unbound antibodies, a substrate is added, which is converted into a colored product by the enzyme bound to the antibody. The amount of colored product produced is directly proportional to the amount of antibodies or antigens present in the initial sample. This method is used in a variety of diagnostic and research applications, including the detection of infectious diseases, cancers, allergies, and other autoimmune diseases. Short version available: • Learn all about the method...

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