Electron transport chain and Chemiosmosis (ATP synthesis) [Oxidative phosphorylation]
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#Biochemistry #ATP #Metabolism ►I explain in detail the stages of oxidative phosphorylation. ►Content: 0:00 Introduction 0:52 Electron transport chain 5:26 Observations of the electron transport chain 6:40 Chemiosmosis (ATP synthesis) 9:02 ATP yield 12:14 Summary ***At minute 09:21 3-5 ATPs should have been written. I wrote NADH instead of ATP. Apologies. ►Additional data -3 H+ pass through the ATP synthase and 1 H+ passes through a transporter that also allows the entry of inorganic phosphorus that will be used for the synthesis of ATP. We could say that for each ATP, 4 H+ have to pass into the mitochondrial matrix. -The ATP synthase rotates COUNTERCLOCKWISE when looking at it from the intermembrane space. -Complex I may also play an important role in programmed cell death or apoptosis. -Complex II or succinate dehydrogenase is part of the Krebs cycle (the only membrane-bound enzyme in the Krebs cycle). We could say that it has a dual role. -An uncoupling agent dissociates the electron transport chain with chemiosmosis, thus preventing the formation of ATP. -NADH and FADH can also come from the catabolism of fatty acids, amino acids, and other extramitochondrial sources. ►Sources used for the preparation of the video: -Ahmad M, et al. Biochemistry, Electron Transport Chain. Treasure Island (FL): StatPearls Publishing; 2021. Available at: https://www.ncbi.nlm.nih.gov/books/NB... -Zhao RZ, et al. Mitochondrial electron transport chain, ROS generation and uncoupling (Review). International journal of molecular medicine. 2019;44(1):3-15. Available at: https://www.ncbi.nlm.nih.gov/pmc/arti... -Nolfi-Donegan D, et al. Mitochondrial electron transport chain: Oxidative phosphorylation, oxidant production, and methods of measurement. Redox Biology. 2020;37:101674. Available at: https://www.ncbi.nlm.nih.gov/pmc/arti... -Deshpande OA, Mohiuddin SS. Biochemistry, Oxidative Phosphorylation. Treasure Island (FL): StatPearls Publishing; 2021. Available at: https://www.ncbi.nlm.nih.gov/books/NB... -Kalpage HA, et al. Cytochrome c phosphorylation: Control of mitochondrial electron transport chain flux and apoptosis. Int J Biochem Cell Biol. 2020;121:105704. Available at: https://www.ncbi.nlm.nih.gov/pmc/arti... ►SUBSCRIBE for more content.
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