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Beer-Lambert's law highlights the proportional relationship between the absorbance and the concentration of a solution. In this video we detail this Beer-Lambert law and explain how to use it when plotting a calibration curve to determine the concentration of an unknown solution. 00:00 Introduction 00:35 Mathematical formula 01:25 Calibration curve 02:04 Determine the concentration of an unknown solution To understand the concept of absorbance and the use of a spectrophotometer: • ABSORBANCE- Spectrophotometer - Like... High school program - 1st specialty physics chemistry: 📝Theme: Constitution and transformations of matter Sub-theme: 1. Determination of the composition of the initial system using physical quantities ✔️ Explain or predict the color of a species in solution from its UV-visible spectrum. Find other videos and methodological sheets to download for free: https://eprofs.fr Twitter: / e_profs Instagram: / e_profs_physique_chimie Facebook: / e-profs-467482426970398